Mid-Temperature Alpha-Amylase is widely used in starch sugar, alcohol, brewery, textile, dye & printing and papermaking,etc.

The enzyme hydrolyzes the α-1,4-glucosidic linkages of gelatinized starch randomly to soluble dextrins, reducing the high viscosity of the starch slurry.

1. Temperature: Optimum 60-70°C,operable up to 90°C . 
2. PH: 6.0-7.0, Optimum PH 6.0. It is deactivated at below pH5.0. 
3. Ca 2 +: required.

1. Solid type: 2000, 3000, 3500, 4000u/g. Premium grade: 4000u/g, 6000u/g, 8000u/g, and 10000u/g. Liquid type: 1500, 2000, 2500, 3000u/ml,4000u/ml 
2. Unit definition : The amount of enzyme needed to hydrolyze 1mg of starch in one hour at pH6.0, 60 °C .

1. Starch sugar and gourmet powder: Concentration of starch syrup 16-17B , pH 6.2-6.4 , CaCl2 0.2%, add the enzyme in the rate of 6-8U/g, stir and heat to 85-90°C, keep for 30 minutes. 
2. In brewing of beer, add the enzyme in the rate of 6U/g, keep at 85-90°C for 30 minutes. 
3. In textile desizing, add the enzyme in the rate of 0.2%(2000u/g), keep at 50-80 °C for 20-40 minutes. 
4. Others: Rate of use is generally 6-8U/g, Ca 2 + concentration 150ppm.

Plastic weave bag is used in solid type, 20kg/bag: Plastic drum is used in liquid type, 25kg/drum. It shall be kept in a cool dry place. Avoid direct sunlight.

Medium temperature alpha-Amylase is a high-performance biological enzyme designed for the efficient hydrolysis of starch-based substrates under moderate temperature conditions. It is widely used in industries such as starch sugar production, alcohol fermentation, brewing, textile desizing, dyeing, printing, and papermaking. The medium temperature alpha-Amylase effectively catalyzes the hydrolysis of α-1,4-glucosidic bonds in gelatinized starch, converting it into soluble dextrins and reducing the viscosity of starch slurries, which greatly improves process efficiency and product quality.

This medium temperature alpha-Amylase exhibits optimal activity at temperatures between 60°C and 70°C and remains operable up to 90°C, offering excellent thermal stability across a broad range of industrial applications. It performs best within a pH range of 6.0–7.0, with an optimum at pH 6.0. However, the enzyme becomes inactive at pH levels below 5.0. Calcium ions (Ca²⁺) are essential for maintaining its stability and catalytic activity, ensuring consistent performance in demanding processing environments.
The medium temperature alpha-Amylase is available in both solid and liquid forms, with activity grades ranging from 2000 to 10000u/g for solid types and 1500 to 4000u/ml for liquid types. The enzyme is defined by its ability to hydrolyze 1 mg of starch per hour at pH 6.0 and 60°C. It demonstrates superior performance in starch sugar manufacturing, where it efficiently liquefies starch syrups at high temperatures, as well as in brewing, where it enhances fermentation yield and clarity. In the textile industry, it enables precise and gentle desizing of fabrics, ensuring smooth texture and color consistency without fiber damage.
Packaging options include 20 kg bags for the solid form and 25 kg drums for the liquid form. The medium temperature alpha-Amylase should be stored in a cool, dry environment away from direct sunlight to maintain its enzymatic stability and activity. With its high catalytic efficiency, broad adaptability, and reliable stability, the medium temperature alpha-Amylase is an ideal choice for various starch processing and enzyme-based production systems.